Targeting Specific PDZ Domains of PSD-95: Structural Basis for Enhanced Affinity and Enzymatic Stability of a Cyclic Peptide dayong
Title: Targeting Specific PDZ Domains of PSD-95: Structural Basis for Enhanced Affinity and Enzymatic Stability of a Cyclic Peptide dayong
Category: /Recreation & Sports/Health Care
Details: Words: 3517 | Pages: 13 (approximately 235 words/page)
Targeting Specific PDZ Domains of PSD-95: Structural Basis for Enhanced Affinity and Enzymatic Stability of a Cyclic Peptide dayong
Category: /Recreation & Sports/Health Care
Details: Words: 3517 | Pages: 13 (approximately 235 words/page)
A cyclic peptide, Tyr-Lys-c[-Lys-Thr-Glu(βAla)-]-Val, incorporating a β-Ala lactam side chain linker and designed to target the PDZ domains of the postsynaptic density protein 95 (PSD-95), has been synthesized and structurally characterized by NMR while free and bound to the PDZ1 domain of PSD-95. While bound, the lactam linker of the peptide makes a number of unique contacts outside the canonical PDZ binding motif, providing a novel target for PDZ-domain specificity
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Received: November 21, 2003
Revised: January 8, 2004
Accepted: January 8, 2004
Published: April 16, 2004